restriction enzyme pml i Search Results


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Bacterial strains and plasmids used in this work
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Bacterial strains and plasmids used in this work
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Bacterial strains and plasmids used in this work
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Bacterial strains and plasmids used in this work
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Bacterial strains and plasmids used in this work
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Bacterial strains and plasmids used in this work
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Bacterial strains and plasmids used in this work
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GenScript corporation pg2rnai2 (genbank: kt954097, from 452th to 4450th nucleotide, 5′ end flanked with bspeⅰ and 3′ end flanked with pmlⅰ restriction enzyme sites)
Bacterial strains and plasmids used in this work
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Bacterial strains and plasmids used in this work
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Bacterial strains and plasmids used in this work
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Image Search Results


Bacterial strains and plasmids used in this work

Journal: Scientific Reports

Article Title: Heterologous viral expression systems in fosmid vectors increase the functional analysis potential of metagenomic libraries

doi: 10.1038/srep01107

Figure Lengend Snippet: Bacterial strains and plasmids used in this work

Article Snippet: The metagenomic library was constructed according to the CopyControl™ Fosmid Library Production kit protocol (Epicentre), except that pMPO579 was used instead of pCC1FOS. pMPO579 was linearised by restriction with the Pml I enzyme (New England Biolabs, isoschizomer of Eco 72I), dephosphorylated with Shrimp Alkaline Phosphatase (USB) and concentrated to 300 mg L −1 with a Centrifugal Filter Device (Microcon, Millipore).

Techniques: Acetylene Reduction Assay, Plasmid Preparation, Clone Assay, Expressing, Derivative Assay